Journal: The Journal of comparative neurology

Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

doi: 10.1002/cne.24699

Figure Lengend Snippet: Primary antibodies

Article Snippet: Connexin 37 , (C)EHQMAKISVAEDGR , rabbit , 1:2000 , poly , Alomone, ACC-204 , RRID:AB_2722598.

Techniques: Marker, Purification, Recombinant

Journal: Genes & Development

Article Title: Mechanotransduction activates canonical Wnt/β-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves

doi: 10.1101/gad.282400.116

Figure Lengend Snippet: The canonical Wnt/β-catenin signaling pathway is active in the developing LVVs and LVs. ( A – I ) TCF/LEF-H2BEGFP embryos were collected at E12.0 ( A – C ), E14.5 ( D – F ), and E18.5 ( G – I ). E12.0 and E14.5 embryos were frontally sectioned, and IHC was performed for GFP, PROX1, and the pan-EC marker CD31. The mesenteries of E18.5 embryos were analyzed by whole-mount IHC for the same markers. The canonical Wnt/β-catenin signaling pathway is active in the GFP + cells of the LVVs ( A – F , yellow arrows) and LVs ( G – I , yellow arrow). ( J – N ) The expression of Axin2 , a target of Wnt/β-catenin signaling pathway, was analyzed in E14.5 embryos by in situ hybridization. Adjacent sections were coimmunostained for PROX1. ( J , K ) PROX1 + ECs and the mesenchyme of the semilunar valves of the heart are Axin2 + . ( L – N ) LVVs (arrows) strongly express Axin2 . ( O , P ) The mesenteric lymphatic vessels were immunostained for total β-catenin ( O ) or nonphosphorylated active β-catenin ( P ). LVs are strongly labeled by both antibodies (red arrows). (LS) Lymph sacs; (IJV) internal jugular vein; (SCV) subclavian vein; (SVC) superior vena cava. Bars: A–F , J–P , 100 µm; G – I , 50 µm. n = 4 for each experiment.

Article Snippet: Primary antibodies used for immunostaining were as follows: rabbit anti-PROX1 (AngioBio), goat anti-human PROX1 (R&D Systems), sheep anti-mouse FOXC2 (R&D Systems), goat anti-mouse VEGFR3 (R&D Systems), goat anti-mouse LYVE1 (R&D Systems), rat anti-mouse CD31 (BD Pharmingen), goat anti-mouse GATA2 (R&D Systems), rabbit anti-total β-catenin and rabbit anti-active β-catenin antibodies (both from Cell Signaling Technologies), goat anti-mouse NRP2 (R&D Systems), chicken anti-GFP (Abcam), rat anti-mouse Endomucin (eBioscience), rabbit anti-CX37 (Invitrogen), and Cy3-conjugated monoclonal anti-α-SMA (Sigma-Aldrich).

Techniques: Marker, Expressing, In Situ Hybridization, Labeling

Journal: PLoS ONE

Article Title: Wall Shear Stress Effects on Endothelial-Endothelial and Endothelial-Smooth Muscle Cell Interactions in Tissue Engineered Models of the Vascular Wall

doi: 10.1371/journal.pone.0088304

Figure Lengend Snippet: A summary of published endothelial-endothelial interactions examined in different vascular wall models.

Article Snippet: In order to differentiate the different cells, each sample was labeled for VE-Cadherin (which is positive for HUVEC and negative for SMC) using a mouse anti VE-Cadherin antibody (Santa Cruz) and for one of the following primary antibodies: rabbit anti Cx37 alexa fluor 647 conjugated (BIOS), rabbit anti Cx43 alexa fluor 488 conjugated (BIOS) followed by the secondary antibodies: alexa fluor 647 conjugated goat anti rabbit and alexa fluor 488 or alexa fluor 647 conjugated goat anti mouse.

Techniques: Permeability, Expressing